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Epithelial expression of HLA class II antigens and Feγ receptors in patients with adult periodontitis
Author(s) -
Nunes Ivana Pereira,
Johannessen Anne Christine,
Matre Roald,
Kristoffersen Tore
Publication year - 1994
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1994.tb01168.x
Subject(s) - periodontitis , monoclonal antibody , epithelium , immunofluorescence , connective tissue , gingival margin , pathology , immunohistochemistry , microbiology and biotechnology , basal (medicine) , antigen , chemistry , biology , immunology , medicine , antibody , dentistry , insulin
The distribution of HLA class II (DR, DP, DQ) and FcγR (I, II, III) was analyzed in the epithelia of patients with advanced marginal periodontitis using cryostat sections incubated with monoclonal antibodies (MoAb) against the Langerhans cell (LC) (CDla) and various subtypes of HLA class II and FcγR, and the indirect immunofluorescence technique. In the oral gingival epithelium (OGE), LC were concentrated subjacent to the connective tissue papillae, while in the pocket epithelium (PE), they were most abundant at the gingival margin. HLA‐DP, DQ, and DR stained LC in both OGE and PE. HLA‐DQ+ LC were significantly fewer than DP+ and DR+ LC. HLA‐DR also stained keratinocytes (KC) in the whole extension of both OGE and PE. HLA‐DP was also observed on KC, but not HLA‐DQ. FcγR II stained both LC and focal areas of KC. In PE FCγR II+ LC were concentrated near the bottom of the pocket, while in the OGE, they were concentrated at the gingival margin. FcγR III was present only on KC, especially in the basal and suprabasal layer. The results indicate that the epithelial cells are actively involved in the development and maintenance of the inflammation of periodontal disease.