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Gingival fluid IL‐1 and IL‐6 levels in refractory periodontitis
Author(s) -
Reinhardt Richard A.,
Masada Marvin P.,
Kaldahl Wayne B.,
DuBois Linda M.,
Kornman Kenneth S.,
Choi JeomIl,
Kalkwarf Kenneth L.,
Allison Anthony C.
Publication year - 1993
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1993.tb00348.x
Subject(s) - eikenella corrodens , medicine , refractory (planetary science) , periodontitis , porphyromonas gingivalis , actinobacillus , clinical attachment loss , dentistry , cytokine , periodontal pathogen , incidence (geometry) , gastroenterology , bacteria , biology , genetics , physics , astrobiology , optics
Selected gingival bacteria and cytokine profiles associated with patients who did not respond to conventional periodontal therapy (refractory) were evaluated. 10 subjects with a high incidence of post‐active treatment clinical attachment loss (>2% sites/year lost ≥ 3 mm) were compared to 10 age‐, race‐, and supragingival plaque‐matched patients with low post‐treatment clinical attachment loss (<0.5% sites/year) relative to the following parameters at 2 sites/patient with the deepest probing depths: (1) presence of 3 selected periodontal pathogens ( Actinobacillus antinomycetemcomitans, Porphyromonas gingivalis. Eikenella corrodens) in subgingival plaque as determined by selective culturing, and (2) gingival crevicular fluid (GCF) levels of 3 cytokines associated with bone resorption (IL‐1 alpha, IL‐1 beta, IL‐6) as determined by two‐site ELISA. Results indicated no significant differences in any clinical measurement (except incidence of clinical attachment loss), in the presence of any bacterial pathogen, or in GCF cytokine levels between refractory subject sites versus stable subject sites. However, when sites producing the greatest total GCF cytokine/patient were compared, sites from refractory patients produced significantly more IL‐6 (30.1 ± 4.0 versus 15.4 ± 2.8 nM, p <0.01). The subgingival presence of each of the 3 bacterial pathogens was associated with elevated GCF IL‐1 concentrations. These data suggest that gingival IL‐1 and IL‐6 production is different in response to local and systemic factors associated with periodontitis, and that IL‐6 may play a role in the identification and mechanisms of refractory periodontitis.