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Concentration of metronidazole in periodontal pockets after application of a metronidazole 25% dental gel
Author(s) -
Stoltze Kaj
Publication year - 1992
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1992.tb02531.x
Subject(s) - metronidazole , gingival and periodontal pocket , periodontal disease , dentistry , medicine , chemistry , antibiotics , microbiology and biotechnology , biology
. The metronidazole concentration was monitored in gingival crevicular fluid (GCF) of inflamed periodontal pockets after 1 application of a 25% gel. 12 patients with periodontal disease were included in the study. Metronidazole gel was applied with a syringe into the pockets of 10 teeth with probing depths ≥ 5 mm. Samples of GCF were taken with Periopaper® before application and 4, 8, 12, 24 and 36 h after application. At each time, samples from 2 teeth were pooled in order to obtain a sufficient amount of fluid for analysis. By means of a calibrated Periotron®, the collected volume of GCF was measured. High‐performance liquid chromatography was used to determine the amount of metronidazole. MIC 50 for anaerobic periopathogens susceptible to metronidazole is below 1 μg/ml. In this study, the concentration obtained was higher than 1 μg/ml in all samples after 4 and 8 h, in 92% after 12 h, in 50% after 24 h and in 8% after 36 h. Thus, metronidazole concentrations in the pockets were generally above MIC 50 for susceptible periopathogens 24 h after 1 application of a 25% metronidazole gel.