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A sensitive method for the detection of immune complexes in human gingival crevicular fluid
Author(s) -
Turner D. W.,
Pederson E. D.,
Lamberts B. L.
Publication year - 1992
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1992.tb00690.x
Subject(s) - periodontal disease , immune system , medicine , dentistry , immunology
An assay for immune complexes [ICs] in gingival crevicular fluid [GCF] could be potentially useful to ascertain the risk of periodontal disease activity at specific oral sites, This paper describes and ELISA‐based method sufficiently sensitive to measure ICs in GCF samples at levels below 1 μg/ml. Static GCF samples taken from 5 adults showed IC levels ranging from 5 to 166 μg/ml. Additional tests of 10 GCF samples from 1 adult were conducted with complement components and indicated that either Clq or rabbit anti‐human C3d was suitable as a capture agent in the assay procedure, Further application of this assay may help to assess the usefulness of IC levels in GCF samples as possible diagnostic indicators of periodontal disease activity.