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Granulocyte elastase in gingival crevicular fluid
Author(s) -
Gustafsson A.,
Åsman B.,
Bergström K.,
Söder PÖ
Publication year - 1992
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1992.tb00680.x
Subject(s) - elastase , periodontitis , gingivitis , granulocyte , pancreatic elastase , medicine , immunology , neutrophil elastase , antigen , inflammation , chemistry , enzyme , dentistry , biochemistry
The granulocyte elastase activity and the immuno‐reactive (antigenic) granulocyte elastase of gingival crevicular fluid (GCF) were studied in 16 periodontitis patients and in 10 gingivitis patients. The elastase activity was measured with a low molecular weight substrate specific for granulocyte elastase. The antigenic elastase was determined with specific antibodies against granulocyte elastase. Intracrevicular sampling of GCF with paper strips for 30 s seemed to provide representative values of elastase. The elastase activity correlated with probing depth and attachment loss and appeared to be a measure of the degree of tissue destruction. Antigenic elastase represents the number of granulocytes in GCF and should thus be related to the degree of inflammation. The periodontitis patients and the gingivitis patients both had a similar degree of inflammation as measured by antigenic elastase per μ1 GCF and gingival index. The elastase activity per μ1 GCF. however, was higher in the periodontitis group. Elevated granulocyte elastase activity in GCF seems to be independent of inflammation and could thus be an indicator of patients at risk for periodontitis.