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Determination of lymphocyte populations and subpopulations extracted from chronically inflamed human periodontal tissues
Author(s) -
Malberg K.,
Mölle A.,
Streuer D.,
Gängler P.
Publication year - 1992
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1992.tb00631.x
Subject(s) - collagenase , periodontitis , cd8 , monoclonal antibody , lymphocyte , medicine , cd3 , connective tissue , pathology , immunology , antibody , immune system , biology , biochemistry , enzyme
The lymphocyte populations and subpopulations extracted from inflamed periodontal tissues of patients with adult periodontitis were determined. 34 patients were grouped according to the gingival index score (GI) of 1, 2 and 3. Gingival tissue from 2 involved teeth was excised, treated with collagenase, and infiltrating cells were isolated and identified using monoclonal antibodies for lymphocyte sets and subsets. The % of CD3+ cells was about 54.5% in all 3 patient groups, but the percentage of CD22 + cells increased from 28.9 ± 3.3% in the group with GI = 1 to 33 ± 1.2% in the group with GI = 3. %s of CD4+ cells and activated CD4+ cells increased from 30.2 + 2,1% and 4.1 ±1.1% in the group with GI= 1 to 38.4±1.2% and 16.0 ± 3.4% in the group with GI = 3, respectively, while in the same groups, the % of CD8 + cells decreased from 24.9 ± 2.0% to 17.7 ± 1.6%. These data indicate a possible importance of activated CD4 + cells in pathogenetic mechanisms of periodontitis.