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Superoxide formation and chemiluminescence of peripheral polymorphonuclear leukocytes in rapidly progressive periodontitis patients
Author(s) -
Shapira Lior,
Borinski Ruth,
Sela Michael N.,
Soskolne Aubrey
Publication year - 1991
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1991.tb01118.x
Subject(s) - opsonin , periodontitis , chemiluminescence , superoxide , luminol , radical , phagocytosis , chemistry , pathogenesis , granulocyte , reactive oxygen species , immunology , phorbol , oxygen , medicine , biochemistry , enzyme , organic chemistry , protein kinase c
Previous studies have indicated that in certain types of chronic inflammatory periodontal diseases, polymorphonuclear leucocytes (PMN) functions are impaired. In view of the damage oxygen‐free radicals may cause to the periodontal tissues, the present study focussed on superoxide (SO) formation and luminol‐dependent chemiluminescence (LDCL) by peripheral PMN cells in rapidly progressive periodontitis patients (RPP). PMN cell preparations were stimulated by either opsonized bacteria or phorbol myristate acetate (PMA). The results indicate that PMN cells from RPP patients, stimulated by opsonized bacteria, have significantly enhanced SO formation and LDCL response as compared to healthy subjects. The hyperactivity was cell‐associated. In the presence of PMA, no significant differences were detected between the groups. The results suggest that PMN cells from RPP patients are functionally activated, and produce elevated levels of oxygen radicals. These oxygen radicals may play a role in the pathogenesis of RPP.