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Flow‐cytometric analysis of lymphocyte subsets in patients with advanced periodontitis
Author(s) -
Zafiropoulos G. G. K.,
Jacoby L. Floresde,
Schoop B.,
Havemann K.,
Heymanns J.
Publication year - 1990
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1990.tb01685.x
Subject(s) - periodontitis , flow cytometry , cytotoxic t cell , monoclonal antibody , immunology , t lymphocyte , periodontium , medicine , lymphocyte , lymphocyte subsets , peripheral blood , antibody , t cell , biology , immune system , in vitro , dentistry , biochemistry
Peripheral blood lymphocytes from 36 patients with advanced periodontitis and from 34 healthy subjects were examined using a panel of monoclonal antibodies and fluorescence flow cytometry. The absolute and relative counts of B‐cells, T‐cells, T‐helper (T H ), T‐suppressor/cytotoxic (T S ) cells, activated T‐cells and natural killer cells were assessed and the T H/S ratio was calculated. In the periodontitis patients, the T H/s ratio was 1.56 ± 0.62 and in the controls 1.35 ± 0.55. This was not statistically significant. A classification on the basis of sex also did not show significant differences in the T H/S ratio. B‐cells and activated T‐cells were slightly increased in the periodontitis group. However, all determined lymphocyte subpopulations revealed no significant differences between the groups. These results indicate that local inflammatory reactions and immunoregulatory dysfunction are limited to the periodontium in the patients with advanced periodontitis, with no significant quantitative effects on the peripheral lymphocyte subpopulations.