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Reproducibility of microbiological samples from periodontal pockets
Author(s) -
Dahlén Gunnar,
Renvert Stefan,
Wikström Maude,
Egelberg Jan
Publication year - 1990
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1990.tb01065.x
Subject(s) - reproducibility , bacteroides , dentistry , actinobacillus , medicine , gingival and periodontal pocket , periodontitis , biology , chemistry , chromatography , bacteria , genetics
Duplicate microbiological samples, were taken 1 week apart using the paper point technique from a total of 112 untreated periodontal pockets >6 mm deep in 16 adult periodontal patients. Duplicate samples were also obtained from these sites 6 months following a therapy of oral hygiene instruction and supra‐and subgingival debridement. The reproducibility of the total viable counts and the reproducibility of the proportions of various groups or species of microorganisms were studied from these duplicate samples. The results demonstrated an acceptable degree of reproducibility for the recovery of Actinobacillus actino‐mycetemcomitans and Bacteroides gingivalis. For the total viable counts and for the other investigated bacterial groups, including Bacteroides intermedius , unacceptable levels of reproducibility were observed.