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Neutrophil chemotactic behaviour in patients with early‐onset forms of periodontitis
Author(s) -
Kinane D. F.,
Cullen C. F.,
Johnston F. A.,
Evans C. W.
Publication year - 1989
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1989.tb01649.x
Subject(s) - chemotaxis , periodontitis , agarose , periodontal disease , immunology , medicine , pathology , biology , microbiology and biotechnology , receptor
The locomotory behaviour of peripheral blood neutrophils (PMNs) from patients with juvenile (JP) and rapidly progressive (RPP) forms of early‐onset periodontal disease was studied using the under agarose technique and n‐formyl‐methionyl‐leucyl‐phenylalanine (FMLP) as the chemotractant. PMNs from experimental patients showed normal random, chemotactic and chemokinetic locomotory behaviour when compared with control subjects. Further investigation of single‐cell movements using time‐lapse video analysis also failed to show any significant differences in locomotory behaviour between the PMNs of experimental and control individuals. We conclude that differences in technique may account for much of the variation which exists in the literature with respect to PMN locomotion in periodontal disease. In the final analysis, it is difficult to dispute direct observation of moving cells, and using this approach, we have been unable to confirm the presence of any PMN locomotory defect in our series of patients with early‐onset periodontal disease.