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Chemiluminescence of peripheral polymorphonuclear leukocytes from adult periodontitis patients
Author(s) -
Whyte G. J.,
Seymour Gregory J.,
Cheung Kee,
Robinson Michael F.
Publication year - 1989
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1989.tb01616.x
Subject(s) - periodontitis , fusobacterium nucleatum , medicine , immunology , peripheral blood , fusobacterium , young adult , peripheral , chemiluminescence , opsonin , microbiology and biotechnology , porphyromonas gingivalis , bacteroides , phagocytosis , biology , bacteria , chemistry , organic chemistry , genetics
Polymorphonuclear leukocytes (PMN's) constitute a primary host resistance factor against infection. This study investigated the chemiluminescent (CL) response of peripheral blood PMN's isolated from human subjects with adult periodontitis. 32 subjects were categorized on the basis of age and periodontal disease status into 4 equal groups ‐ young healthy, young diseased, old healthy and old diseased. PMN CL was stimulated using heat‐killed, serum‐opsonized Fusobacterium nucleatum ‐ a specific periodontopathic gram‐negative anaerobe, and Escherichia coli as a gram‐negative control organism. The results showed a statistically significant enhancement ( p < 0.05) in the CL response, which was cell associated, in the young diseased subjects. This was not seen in the old subjects ( p > 0.05), suggesting that in periodontal disease in young subjects the peripheral blood PMNs may be in a metabolically activated state. There was nevertheless a degree of variability between individual subjects within each of the 4 clinical groups.