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Blastogenetic responsiveness of peripheral blood lymphocytes from patients with adult periodontitis, evaluated by a reverse hemolytic plaque assay
Author(s) -
Kopp W.,
Marggraf E.
Publication year - 1986
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1986.tb02234.x
Subject(s) - periodontitis , medicine , fibroblast , stimulation , immune system , virus quantification , pokeweed mitogen , immunology , cytoplasm , antibody , peripheral blood , pathology , peripheral blood mononuclear cell , biology , in vitro , biochemistry , virus
. Peripheral blood lymphocytes were obtained from 10 patients with advanced periodontitis and from 5 normal control subjects. The lymphocytes were cultured with dental plaque, supernatant of homologous fibroblast cytoplasm and Pokeweed‐mitogen (PWM). The number of immunoglobulin‐ secreting cells (ISC) was assessed after 7 days using a reverse hemolytic plaque assay (RHPA). The stimulation levels of lymphocytes from periodontitis patients and control subjects stimulated with dental plaque and PWM were not statistically different ( p >0.05). In contrast, the number of ISC produced after stimulation with fibroblast cytoplasm was increased significantly in the patients group ( p <0.05). These data support the hypothesis that autologous substances play an important rôole in the cellular immune responses of patients with severe chronic inflammatory periodontal disease (CIPD).