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Lactate dehydrogenase activity in gingival crevicular fluid collected with filter paper strips: analysis in subjects with non‐inflamed and mildly inflamed gingiva
Author(s) -
Lamster Ira B.,
Mandella Rosemary D.,
Gordon Jeffriv M.
Publication year - 1985
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/j.1600-051x.1985.tb01374.x
Subject(s) - medicine , lactate dehydrogenase , dentistry , chemistry , enzyme , biochemistry
A previous study of lactate dehydrogenase (LDH Jin gingival crevicular fluid (GCF) suggested that the concentration is 10 to 25 times that of serum (X=2300 international units/I versus 100 IU/1 for serum). That study used capillary tubes to collect microliter amounts of GCF. Since invasive collection techniques can influence GCF flow, we evaluated LDH activity in GCF collected by filter strips. GCF was collected in a standardized fashion from 10 subjects with mild inflammation (GI=0.5–1.0) and 10 subjects without evidence of gingival inflammation (GI=0). Our results indicate that LDH volume activity was greater for subjects with GI=0 x= 105.529 IV/l) than for subjects with GI=0.5–1.0 (X;=77,66 1 IU/1). but the difference was not significant. LDH total unit activity was significantly greater in subjects with GI=0.5–1.0 versus GI=0 (X=0.048 IU versus X=0.0242 IU. P <0.0001). The relationship of LDH volume activity to GCF volume, the regression lines fit to the data, and calculation of LDH total unit activity were important for analysis of enzyme activity in GCF.