Premium
Bi‐layered collagen nano‐structured membrane prototype (collagen matrix 10826 ® ) for oral soft tissue regeneration: an “ in vitro ” study
Author(s) -
Nocini Pier Francesco,
Zanotti Guglielmo,
Castellani Roberto,
Grasso Silvia,
Cristofaro Maria Giulia,
Santis Daniele
Publication year - 2013
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2012.02427.x
Subject(s) - keratinocyte , cytokeratin , adhesion , in vitro , regeneration (biology) , microbiology and biotechnology , matrix (chemical analysis) , tissue engineering , extracellular matrix , biocompatibility , type i collagen , chemistry , cell adhesion , biomedical engineering , immunology , pathology , biology , medicine , biochemistry , immunohistochemistry , organic chemistry , chromatography
Aim To evaluate fundamental cell functions, such as adhesion, IL ‐6 production and proliferation of human gingival keratinocytes cultured on a newly engineered collagen matrix ( CM ‐10826) and to assess the degree of specific biocompatibility of this new device. Materials and methods Primary cultures of human keratinocytes were derived “ in vitro ” from biopsies of independent donors. Their true epithelial origin was ensured by the expression of cytokeratin 14. Adhesion, proliferation and production of IL ‐6 cytokine was then measured in the presence or absence of CM ‐10826 activity or of its relevant components. Results Functional tests revealed that keratinocytes adhered to CM ‐10826 and up‐regulated their basal IL ‐6 production. The type of keratinocytes used expressed cytokeratin 14. Proliferation experiments demonstrated that the best cellular response was observed in the presence of Collagen I, the main component of CM ‐10826. No undesired effects were observed as for keratinocyte viability, morphology or differentiation. Conclusions Our results demonstrate that CM ‐10826 has a favourable biological effect on the “in vitro” response of gingival keratinocytes in terms of IL ‐6 production, cell growth and adhesion. These findings may encourage a possible use of this collagen membrane as a tissue which, alone, may substitute for autologous gingival grafts thereby overcoming the limitations of autologous tissue.