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Biological response of human bone marrow mesenchymal stem cells to fluoride‐modified titanium surfaces
Author(s) -
Guida Luigi,
Annunziata Marco,
Rocci Antonio,
Contaldo Maria,
Rullo Rosario,
Oliva Adriana
Publication year - 2010
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2010.01929.x
Subject(s) - mesenchymal stem cell , human bone , titanium , fluoride , bone marrow , materials science , chemistry , medicine , pathology , in vitro , metallurgy , biochemistry , inorganic chemistry
Objectives: The aim of the present study was to examine the behaviour of human bone marrow‐derived mesenchymal stem cells (BM‐MSC) to fluoride‐modified grit‐blasted (F‐TiO) titanium surfaces compared with grit‐blasted ones (TiO). Material and methods: Implant surfaces were analysed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). BM‐MSC were isolated from healthy donors and grown on the implant surfaces. Cell adhesion and proliferation, type I collagen (Col I) synthesis, osteoblastic differentiation (in terms of alkaline phosphatase activity, osteocalcin synthesis and extracellular matrix mineralization) were assessed. Furthermore, the ability to affect the osteoblastic/osteoclastic balance in terms of osteoprotegerin (OPG) and activator of nuclear factor κ B ligand (RANKL) ratio was investigated. Results: F‐TiO surface showed higher S a values ( P <0.05) and the presence of nano‐scale structures at the AFM and SEM analysis. Comparable cell morphology and similar adhesion values on both surfaces were detected at early time, whereas higher proliferation values on F‐TiO samples were observed at 7 and 10 days. Increased Col I and OPG levels for cells grown on F‐TiO were found, whereas RANKL was not detectable in any of the conditioned media. BM‐MSC showed a similar expression of early and late osteogenic markers on both TiO and F‐TiO surfaces. Conclusions: The results of the present study show that the chemical and micro/nano‐scale modifications induced by fluoride treatment of TiO‐grit blasted surfaces stimulate the proliferation and the extracellular matrix synthesis by BM‐MSC, as well as the increase of OPG synthesis, thus preventing osteoclast activation and differentiation. To cite this article:
Guida L, Annunziata M, Rocci A, Contaldo M, Rullo R, Oliva A. Biological response of human bone marrow mesenchymal stem cells to fluoride‐modified titanium surfaces.
Clin. Oral Impl. Res . 21 , 2010; 1234–1241.
doi: 10.1111/j.1600‐0501.2010.01929.x