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Maxillary sinus floor elevation using a tissue‐engineered bone complex with OsteoBone ™ and bMSCs in rabbits
Author(s) -
Sun X.Juan,
Zhang Z.Yuan,
Wang S.Yi,
Gittens S. A.,
Jiang X.Quan,
Chou L. Lee
Publication year - 2008
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2008.01577.x
Subject(s) - maxillary sinus , stromal cell , bone marrow , mineralization (soil science) , dentistry , medicine , iliac bone , chemistry , surgery , pathology , organic chemistry , nitrogen
Objectives: To evaluate the effects of maxillary sinus floor elevation by a tissue‐engineered bone complex with OsteoBone ™ and bone marrow stromal cells (bMSCs) in rabbits. Material and methods: Autologous bMSCs from adult New Zealand rabbits were cultured and combined with OsteoBone ™ at a concentration of 20 × 10 6 cells/ml in vitro . Twenty‐four animals were used and randomly allocated into groups. For each time point, 16 maxillary sinus floor elevation surgeries were made bilaterally in eight animals and randomly repaired by bMSCs/material (i.e. OsteoBone ™ ), material, autogenous bone and blood clot ( n =4 per group). A polychrome sequential fluorescent labeling was also performed post‐operatively. The animals were sacrificed 2, 4 and 8 weeks after the procedure and evaluated histologically as well as histomorphometrically. Results: New bone area significantly decreased from weeks 2 to 8 in the blood clot group, while bone area in the autologous bone reduced from weeks 4 to 8. In both groups, a significant amount of fatty tissue appeared at week 8. Accordingly, augmented height in both groups was also significantly decreased from weeks 2 to 8. The bone area in the material‐alone group as well as in the bMSCs/material group, on the other hand, increased over time. Significantly more newly formed bone area and mineralization was observed in the center of the raised space in the bMSCs/material group than in the material‐alone group. The augmented height was maintained in these two groups throughout the course of this study. Conclusion: These results suggest that OsteoBone ™ can successfully be used as a bone graft substitute and that the combination of this material with bMSCs can effectively promote new bone formation in sinus elevation.