Premium
In vitro osteogenesis induced by cells derived from sites submitted to sinus grafting with anorganic bovine bone
Author(s) -
Beloti Marcio Mateus,
Martins Walter,
Xavier Samuel Porfirio,
Rosa Adalberto Luiz
Publication year - 2008
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2007.01420.x
Subject(s) - trephine , alkaline phosphatase , bone grafting , chemistry , viability assay , in vitro , maxillary sinus , dentistry , cell counting , grafting , alizarin red , andrology , cell , microbiology and biotechnology , surgery , pathology , medicine , biology , enzyme , staining , biochemistry , cell cycle , organic chemistry , polymer
Objectives: This study evaluated key parameters of the in vitro osteogenesis induced by osteoblastic cells obtained from sites submitted to sinus grafting with anorganic bovine bone (ABB) in comparison with cells derived from bone sites of the same patients. Materials and methods: In three patients, the augmentation of maxillary sinus was carried out using ABB (Bio‐Oss ® ). After at least 6 months, during the surgical intervention for titanium implants placement, biopsies were taken from these areas using trephine burs (grafted group). Bone fragments, of the same patients, from sites that had not received graft were also obtained with trephine burs and used as a control group. Osteoblastic cells were obtained from grafted and control groups by enzymatic digestion and cultured under standard osteogenic condition until subconfluence. First passaged cells were cultured in 24‐well culture plates. Cell adhesion was evaluated at 24 h. For proliferation and viability assay, cells were cultured for 1, 3, 7, and 10 days. Total protein content and alkaline phosphatase (ALP) activity were measured at 3, 7, 10, 14, 17, and 21 days. Cultures were stained with Alizarin red S at 21 days, for detection of mineralized matrix. Data were compared by Student's t ‐test. Results: Cell adhesion and viability were not affected by cell source ( P >0.05). Total protein content was greater ( P <0.05) for grafted group. Cell proliferation, ALP activity, and bone‐like nodule formation were all greater ( P <0.05) for the control group. Conclusion: Taken together, these results indicate that the in vivo long‐term contact of cells with ABB downregulates the expression of osteoblast phenotype and consequently the in vitro osteogenesis.