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Clinical application of tissue‐engineered transplants. Part I: mucosa
Author(s) -
Sauerbier Sebastian,
Gutwald Ralf,
WiedmannAlAhmad Margit,
Lauer Günter,
Schmelzeisen Rainer
Publication year - 2006
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2006.01229.x
Subject(s) - medicine , dentistry , pathology
Objectives: The study series aims at testing the feasibility of the clinical application of tissue‐engineered oral mucosa. The preliminary results were gathered over a period varying from 6 months to 12 years depending on the surgical method. Methods: Tissue‐engineered oral mucosa was used to cover defects in various surgical procedures like vestibuloplasty ( n =42), freeing of the tongue ( n =10), prelaminating the radial flap ( n =5) and reconstruction of the urethra ( n =16). In all interventions small samples of oral mucosa were harvested, cut into small pieces, resuspended in culture medium and seeded into a culture flask. Cultured keratinocytes were transferred onto membranes which then were used to cover mucosal defects in the oral cavity. Results: To gain a graft of 15 cm 2 size a mucosa biopsy of 4–8 mm 2 and 40 ml autologous patients serum is needed. Tissue‐engineered oral mucosa was applied successfully in all four surgical methods. Six months after transplantation a regular epithelial layering with a histological delimitation of the stratum, epithelial crest and a strong basal membrane appeared. According to the reception site the tissue engineered oral mucosa differentiated in several ways. Conclusion: Tissue‐engineered oral mucosa fulfils the requirements for clinical routine. With view to healing time and outcome it does not appear to be superior to regular harvested oral mucosa transplants. Because of a smaller harvesting defect and primary wound closure at the actual operation site the patients' convenience is increased. Thus this method reduces morbidity and advances the quality of life.