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Effect of systemic administration of nicotine on healing in osseous defects. An experimental study in rabbits. Part II
Author(s) -
Balatsouka Dimitra,
Gotfredsen Klaus,
Sørensen Lars,
Lindh Christian H.,
Berglundh Tord
Publication year - 2006
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2005.01248.x
Subject(s) - medicine , nicotine , saline , bone healing , tibia , systemic administration , body weight , prostaglandin , surgery , anesthesia , in vivo , microbiology and biotechnology , biology
Objectives: The aim of the present study was to analyze the effect of systemic administration of nicotine on bone healing in osseous defects in the tibia of rabbits. Material and methods: Sixteen female rabbits received nicotine ( n =8; test group) or saline ( n =8; control group) via subcutaneously placed mini‐osmotic pumps for 8 weeks. The animals underwent three surgical operations during the experimental period, and body weight was registered weekly. Blood samples were collected to determine cotinine and prostaglandin E 2 levels. Bone preparations were made in the right leg of all rabbits after 4 weeks and in the left leg after 6 weeks of nicotine/placebo exposure. Thus, 2‐ and 4‐week healing groups were created for the bone defects. After 8 weeks, the animals were killed. Tissue blocks including the bone defects were prepared for histological analysis. Results: The animals in the test group lost weight, while the control group gained weight during the experiment. The prostaglandin E 2 levels in plasma increased significantly following nicotine exposure in the test group. No significant differences in the percentage of vessels and bone density in the osseous defects were found between the test and the control groups after 2 and 4 weeks of healing. Conclusions: In this experiment, systemic administration of nicotine over 4 or 6 weeks, respectively, influenced body weight and systemic prostaglandin E 2 levels but not the amount of blood vessels and the bone mineral density in bone defects after 2 or 4 weeks of healing.