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Laser Therapie beeinflusst die initiale Anhaftung und die darauf folgende Proliferation von humanen oralen Fibroblasten, welche auf Implantatmaterial aus Titan gezüchtet werden.
Eine rasterelektronenmikroskopische und histomorphometrische Analyse
Author(s) -
Khadra Maawan,
Kasem Nesrin,
Lyngstadaas Ståle Petter,
Haanæs Hans R.,
Mustafa Kamal
Publication year - 2005
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2004.01092.x
Subject(s) - titanium , irradiation , implant , materials science , viability assay , fibroblast , in vitro , cell , andrology , chemistry , biophysics , biomedical engineering , medicine , biology , surgery , metallurgy , biochemistry , physics , nuclear physics
The aim of the study was to investigate the effect of low‐level laser therapy (LLLT) on attachment and proliferation of human gingival fibroblasts (HGF) cultured on titanium implant material. HGF were exposed to gallium–aluminum–arsenide diode laser at dosages of 1.5 or 3 J/cm 2 and then cultured on commercially pure titanium discs. Cell profile areas were measured after 1, 3 and 24 h, using scanning electron microscopy and an automatic image analyzer. The results were expressed as percentage of attachment. In order to investigate the effect of LLLT on cellular growth after 8 and 10 days, HGF were cultured on titanium discs for 24 h and then exposed to laser irradiation on 3 consecutive days. Colony‐forming efficiency (CFE) and clonal growth rates (CGR) were measured. Cell viability was determined by Hoechst and prodidium iodide staining. Non‐lased cultures served as controls. Morphologically, the cells spread well on all titanium surfaces, indicating good attachment by both irradiated and non‐irradiated cells. Fibroblasts exposed to laser irradiation had significantly higher percentages of cell attachment than the non‐exposed cells ( P <0.05). CFE and CGR were also enhanced for the irradiated cells ( P <0.05). Cell viability was high (>90%) in the irradiated and control groups, without significant differences. It is concluded that in vitro LLLT enhances the attachment and proliferation of HGF on titanium implant material.