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The use of porous calcium phosphate scaffolds with transforming growth factor beta 1 as an onlay bone graft substitute
Author(s) -
Huse Robert O.,
Quinten Ruhe P.,
Wolke Johannes G. C.,
Jansen John A.
Publication year - 2004
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2004.01068.x
Subject(s) - biomaterial , calcium , osteoid , calcium phosphate cement , biomedical engineering , implant , cement , bone grafting , in vivo , grafting , porosity , bone tissue , chemistry , dentistry , materials science , surgery , medicine , anatomy , biology , metallurgy , composite material , microbiology and biotechnology , polymer
Objectives: Autogeneous bone grafting is regarded to be the golden standard for onlay grafts, but it requires a harvesting procedure and the remodeling pattern over time is unpredictable. New materials are constantly being sought to overcome these problems. An in vivo experiment was carried out to evaluate whether (1) porous calcium phosphate cement is a suitable biomaterial for onlay bone grafting, and (2) the addition of transforming growth factor beta 1 (TGF‐β1) accelerates de novo bone formation inside the cement porosity. Material and methods: A carrier of porous calcium phosphate cement (Calcibon®) was designed and 16 rats received one preshaped implant each. In 8 out of 16 implants 0.75 μg TGF‐β1 was applied. The animals were killed after 4 weeks and the characteristics of tissue ingrowth into the onlay graft were evaluated. Results: Histologic and quantitative histomorphometrical measurements demonstrated osteoid‐like tissue formation in both experimental groups. The addition of TGF‐β1 did not induce significantly more osteoid‐like tissue formation. On the other hand, in TGF‐β‐loaded implants, a higher number of pores contained an inflammatory infiltrate. Conclusion: This study indicated that porous calcium phosphate cement is a promising material for clinical situations where bone formation has to be supported.