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Die Effekte von Thrombozyten‐Lysaten auf ausgewählte Funktionen von Knochenzellen
Author(s) -
Soffer Emmanuel,
Ouhayoun JeanPierre,
Dosquet Christine,
Meunier Alain,
Anagnostou Fani
Publication year - 2004
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2004.01063.x
Subject(s) - platelet , calvaria , chemistry , collagenase , andrology , alkaline phosphatase , platelet rich plasma , calcium , osteocalcin , in vitro , endocrinology , medicine , biochemistry , biology , enzyme , organic chemistry
Abstract: Although platelet‐rich plasma and platelet concentrates have been used to promote bone healing in orthopaedic and maxillofacial surgery, the underlying cellular‐level mechanisms remain poorly understood. The present in vitro study investigated the effects of human platelet lysate (PL) on selected functions of cultured bone cells. Cells from 18‐day‐old fetal rat calvaria were isolated by a collagenase digestion procedure. PL was added at different concentrations on pre‐ or post‐confluent cell stage. After 1 day, bone cellproliferation was maximal and half‐maximal in the presence of PL from 3 × 10 8 and 0.5 × 10 8 platelets/ml, respectively. During 17 h, the number of bone cells traversing the scrape border of a scrape wound model increased by 16‐fold in the presence of PL from 3 × 10 8 platelets/ml. The presence of PL from 3 × 10 8 platelets/ml in pre‐confluent bone cellcultures for 48 h resulted in a threefold decrease of alkaline phosphatase (ALP) specificactivity. In the case of confluent bone cells, the presence of PL (from 1 × 10 6 to 3 × 10 8 platelets/ml) for 11 days, the ALP specific activity and total calcium content decreased in a PL dose‐dependent manner and reached a minimum in the presence of PL from 3 × 10 8 platelets/ml. In summary, short‐term PL exposure (up to 24 h) promotes the proliferative and chemotactic bone cell functions while long‐term PL exposure results in adecrease of both ALP activity and mineral formation. These data show that the soluble components contained in PL may affect the bone healing process by modulating differently bone cell functions.