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Die Analyse von MG63 osteoblastenähnlichen Zellen auf einer nanoporöse Implantatoberfläche mittels einer Mikroordnungstechnologie
Author(s) -
Carinci Francesco,
Pezzetti Furio,
Volinia Stefano,
Francioso Francesca,
Arcelli Diego,
Marchesini Jlenia,
Caramelli Elisabetta,
Piattelli Adriano
Publication year - 2004
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/j.1600-0501.2004.00997.x
Subject(s) - dna microarray , osseointegration , implant , microarray analysis techniques , gene , gene expression profiling , microbiology and biotechnology , microarray , gene expression , osteoblast , biology , materials science , genetics , medicine , in vitro , surgery
Surface implant modifications have been shown to have a relevant importance in modifying cell response. Expression profiling by DNA microarray is a new molecular technology that allows the analysis of gene expression in a cell system. By using DNA microarrays containing 19,200 genes, we identified in osteoblast‐like cells line (MG‐63) on new implant surface (nanoPORE, Out‐Link, Sweden and Martina, Due Carrare, Padova, Italy), several genes whose expressions were significantly down‐regulated. The differentially expressed genes cover a broad range of functional activities: (a) immunity, (b) vesicular transport (c) apoptosis and cell cycle regulation. It was also possible to detect some genes whose function is unknown. The data reported are, to our knowledge, the first genetic portrait of an implant surface. They can be relevant to better understand the molecular mechanism of implant osseointegration and as a model for comparing other materials.