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Enhancement of immune response induced by DNA vaccine cocktail expressing complete LACK and TSA genes against Leishmania major
Author(s) -
Ghaffarifar Fatemeh,
Jorjani Ogholniaz,
Sharifi Zohreh,
Dalimi Abdolhossein,
Hassan Zuhair M.,
Tabatabaie Fatemeh,
Khoshzaban Fariba,
Hezarjaribi Hajar Ziaei
Publication year - 2013
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2012.02968.x
Subject(s) - immune system , dna vaccination , gene , dna , leishmania , leishmania major , chemistry , biology , microbiology and biotechnology , immunology , genetics , immunization , parasite hosting , world wide web , computer science
Leishmaniasis is an important disease in humans. Leishmania homologue of receptor for Activated C Kinase ( LACK ) and thiol specific antioxidant ( TSA ) as immuno‐dominant antigens of Leishmania major are considered the most promising molecules for a DNA vaccine. We constructed a DNA cocktail, containing plasmids encoding LACK and TSA genes of Leishmania major and evaluated the immune response and survival rate in BALB /c mice. IgG and Interferon gamma values were noticeably increased in the immunized group with DNA cocktail vaccine, which were significantly higher than those in the single‐gene vaccinated and control groups (p < 0.05) following the immunization and after challenging with Leishmania major . Interleukin 4 values were decreased in all immunized groups, but only in DNA vaccine cocktail and single‐gene vaccination with pc‐ LACK there were statistical differences with control groups (p > 0.05 ) . The immunized mice with the cocktail DNA vaccine presented a considerable reduction in diameter of lesion compared to other groups and a significant difference was observed (p < 0.05) in this regard. The survival time of the immunized mice with the cocktail DNA vaccine was significantly higher than that in the other groups (p < 0.05) after their being challenged with Leishmania major . The findings of this study indicated that the cocktail DNA vaccine increased the cellular response and survival rate and induced protection against infection with Leishmania in the mice.