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Immunohistological expression of HIF ‐1α, GLUT ‐1, B cl‐2 and K i‐67 in consecutive biopsies during chemoradiotherapy in patients with rectal cancer
Author(s) -
Havelund Birgitte Mayland,
Sørensen Flemming Brandt,
Pløen John,
Lindebjerg Jan,
Spindler KarenLise Garm,
Jakobsen Anders
Publication year - 2013
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2012.02949.x
Subject(s) - medicine , pathological , chemoradiotherapy , colorectal cancer , tegafur , adenocarcinoma , immunohistochemistry , carcinoma , gastroenterology , cancer , pathology
The aim of this study was to describe the dynamics of HIF ‐1α, GLUT ‐1, Bcl‐2 and Ki‐67 during chemoradiotherapy ( CRT ) of rectal cancer, and to investigate the fluctuation of these biomarkers in relation to pathological response to CRT . The study included 86 patients with rectal adenocarcinoma receiving preoperative CRT (>50.4 Gy and Uracil/Tegafur). Immunohistological expressions of HIF ‐1α, GLUT ‐1, Bcl‐2 and Ki‐67 were investigated in biopsies taken before treatment, after 2, 4 and 6 weeks of CRT and in specimens from the operation. Decreasing expressions of HIF ‐1α, Bcl‐2 and Ki‐67 were observed during CRT , whereas GLUT ‐1 overall was unchanged. No significant changes of the markers were observed in the interval between CRT and surgery. A significant association was observed between the presence of residual carcinoma after 6 weeks of treatment and pathological response to CRT , but no association was seen between the fluctuations of any of the markers and response to CRT . This unique material containing specimens before, after and during CRT for rectal cancer demonstrated biological dynamics in HIF ‐1α, Bcl‐2 and Ki‐67, but not GLUT ‐1, expression during CRT , and a significant association was seen between the presence of residual carcinoma after 6 weeks of treatment and pathological response to CRT .