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Antibiotic resistance and molecular epidemiology of the beta‐lactamase‐producing H aemophilus influenzae isolated in Chongqing, China
Author(s) -
Luo Chong,
Xia Yongwu,
Liu Qian,
Chu Lijuan,
Fu Xiaohong,
Jing Chunmei,
Chen Dapeng,
Liu Lan,
Shi Yu
Publication year - 2012
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2012.02921.x
Subject(s) - ampicillin , microbiology and biotechnology , amp resistance , haemophilus influenzae , antibiotics , molecular epidemiology , antibiotic resistance , biology , genotype , virology , gene , genetics
This study aimed to determine the antibiotic resistance and molecular epidemiology of H aemophilus influenzae isolated from children with acute respiratory infection in Chongqing, China. To this end, 1967 H . influenzae isolates from 2006 to 2009 were analysed regarding β‐lactamase production and antibiotic resistance. Ninety‐nine β‐lactamase‐producing H . influenzae isolates from 2010 were analysed for antibiotic resistance and promoter regions of bla TEM ‐1 . β‐lactamase production was found in 35.8% (705/1967) of the strains. All ninety‐nine β‐lactamase‐producing strains from 2010 were of the TEM ‐1 type as determined by PCR but did not produce the predicted 1075 bp product. According to PCR ‐ SSCP and DNA sequencing, the promoter regions of bla TEM ‐1 were categorized into 6 genotypes as SSCP 1 (Pdel), SSCP 2 (Pa/Pb), SSCP 3 (P4), SSCP 4 (Prpt.b), SSCP 5 (2Prpt) and SSCP 6 (P3.b). The Pdel, Pa/Pb and Prpt.b were common promoters of bla TEM ‐1 for H . influenzae isolated from children in Chongqing. Strains with Prpt.b were more resistant to ampicillin ( AMP ) than strains with Pdel, Pa/Pb and P4 (p < 0.05). Therefore, bla TEM‐1 β‐lactamase is the main mechanism for resistance of H . influenzae to ampicillin in Chongqing. Furthermore, the Prpt.b promoters may be related to the high resistance of H . influenzae to AMP.