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Type III apparatus of P seudomonas aeruginosa as a tool to diagnose pulmonary infection in cystic fibrosis patients
Author(s) -
Cruz Aline C.,
Neves Bianca C.,
Higa Laurinda Y. S.,
Folescu Tânia,
Marques Elizabeth A.,
Milagres Lucimar G.
Publication year - 2012
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2012.02888.x
Subject(s) - cystic fibrosis , pseudomonas aeruginosa , western blot , serology , secretion , antigen , microbiology and biotechnology , blot , antibody , biology , medicine , immunology , bacteria , biochemistry , gene , genetics
Pseudomonas aeruginosa is associated with increased mortality in cystic fibrosis ( CF ) patients, and expresses type III secretion system proteins ( TTSP ), which is a common mechanism used by gram‐negative pathogens for delivery of anti‐host factors. Our aim was to investigate whether or not these antigens ( TTSP ) would be recognized by CF sera, by Western blot reaction. We have showed herein that all patients (n = 11) not chronically infected by P . aeruginosa had their first serum positive for TTSP ( ExoS , ExoT , PopB , and/or PopD ). All chronic patients had a strong positive serology to TTSP , although relatively weak reactions to TTSP were observed for some individuals in the negative control group. Therefore, TTSP that were early produced in P . aeruginosa infected CF patients, induced a detectable antibody response in those patients and were easily detected by Western‐blot reaction.