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Increased detection of cutaneous leishmaniasis in N orway by use of polymerase chain reaction
Author(s) -
Blonski Katharina M.,
BlödornSchlicht Norbert,
Falk Thomas M.,
Faye Ragnar S.,
Clausen Ole P. F.
Publication year - 2012
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2012.02875.x
Subject(s) - histopathology , cutaneous leishmaniasis , leishmaniasis , polymerase chain reaction , medicine , pathology , dermatology , biopsy , leishmania , skin biopsy , histopathological examination , clinical diagnosis , biology , parasite hosting , clinical psychology , biochemistry , world wide web , computer science , gene
Cutaneous leishmaniasis (CL) is a parasitic infection and occurs in tropical and subtropical regions worldwide and in the region of the Mediterranean Sea. The diagnosis is based on the clinical appearance and biopsy findings that may be supplemented with polymerase chain reaction (PCR). In this study 20 cases were selected if (i) a histopathological diagnosis of granulomatous dermatitis was made, (ii) CL was taken into consideration or (iii) the diagnosis was CL. PCR analysis with primers specific for leishmania was performed on archived histological specimens and was positive in 6 of the 20 cases. In two cases both the clinical and histopathological diagnosis concurred with CL. In the remaining four cases a clinical diagnosis other than CL was made. In two of these cases the histopathology showed granulomatous dermatitis, and detection of parasites led to consideration of CL. In the last two cases leishmaniasis was not taken into consideration by clinicians or pathologists. Our study shows that CL may occur more often than anticipated in Norway, but clinicians do not consider the diagnosis as often as they should. Pathologists may also fail to diagnose or suggest CL especially when parasites are not visualized in the histopathological specimen.