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Galectin‐1 is up‐regulated by RASSF1A gene in human gastric carcinoma cell line SGC7901
Author(s) -
ZhengHao Deng,
JiFang Wen,
DeSheng Xiao,
JianHua Zhou
Publication year - 2012
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2012.02874.x
Subject(s) - vimentin , microbiology and biotechnology , galectin 1 , cell culture , galectin , biology , gene expression , galectin 3 , blot , cancer cell , gene , cancer research , cancer , biochemistry , immunohistochemistry , immunology , genetics
We have previously shown that overexpression of RASSF1A inhibits the growth of human gastric cancer SGC7901 cells, but the underlying mechanism remains unknown. In this study, the differential protein expression by RASSF1A gene in human gastric cancer cell line SGC7901 was determined by 2‐D gel electrophoresis combined with matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry ( MALDI ‐ TOF MS ) and bioinformatics. Differential expression analysis of the protein profiles by RASSF1A gene identified a total of 35 protein spots, of which 10 were up‐regulated and 25 were down‐regulated. Eight proteins were identified by MALDI ‐ TOF MS : Galectin‐1, TRP‐14 , ACBP , PSMB5 , PSMB4 , TIM , vimentin, CD79α . RASSF1A up‐regulated the mRNA expression of G alectin‐1, TRP‐14 , ABCP in SGC7901 . RASSF1A also led to an increased expression of G alectin‐1 protein in SGC7901 confirmed by western blotting and immunocytochemistry analysis. RASSF1A inhibited the activity of NF ‐ κB in SGC7901 cells. These data indicated that G alectin‐1 may be playing a role in RASSF1A signaling in SGC7901 .