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C3b‐induced eosinophil degranulation involves PI3‐kinases and is inhibited by protein kinase C activity
Author(s) -
CARLSON MARIE,
VENGE PER,
LAMPINEN MARIA
Publication year - 2011
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2010.02701.x
Subject(s) - wortmannin , staurosporine , degranulation , eosinophil cationic protein , eosinophil peroxidase , eosinophil , eosinophil granule proteins , kinase , ly294002 , microbiology and biotechnology , protein kinase a , major basic protein , protein kinase c , chemistry , biochemistry , biology , immunology , phosphatidylinositol , receptor , asthma
Carlson M, Venge P, Lampinen M. C3b‐induced eosinophil degranulation involves PI3‐kinases and is inhibited by protein kinase C activity. APMIS 2010; 119: 119–26. Selective release of individual eosinophil granule proteins has been demonstrated in eosinophilic conditions and in vitro using different stimuli. The aim of this study was to investigate if selective release of eosinophil cationic protein (ECP), eosinophil protein X/eosinophil derived‐neurotoxin (EPX/EDN) and eosinophil peroxidase (EPO) could be due to the involvement of different signal transduction pathways. Peripheral blood granulocytes from healthy donors were incubated with Wortmannin, LY294002, Genistein, Staurosporine, GÖ6976 or PD98059 prior to the induction of degranulation by C3b. The released amounts of ECP, EPO and EPX/EDN were determined by immunoassays, and related to the total cell content of respective protein. Wortmannin caused a significant, dose‐dependent inhibition of all three granule proteins. LY294002 (10 −6 M) also inhibited the release of all proteins. Genistein (10 −6 M) inhibited the release of ECP, whereas the release of EPO was increased. However, there was a tendency towards similar concentration‐dependent patterns of release of all three proteins. Staurosporine (10 −7 M), GÖ6976 (10 −6 M) and PD98059 (10 −5 M) caused an increased release of the three proteins. PI3‐kinases play an important role in the C3b‐induced release of ECP, EPO and EPX/EDN, whereas protein kinase C seems to have inhibitory effects on C3b‐induced degranulation.