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A porcine model of acute, haematogenous, localized osteomyelitis due to Staphylococcus aureus : a pathomorphological study
Author(s) -
JOHANSEN LOUISE KRUSE,
FREES DORTE,
AALBÆK BENT,
KOCH JANNE,
IBURG TINE,
NIELSEN OLE LERBERG,
LEIFSSON PALL SKULI,
JENSEN HENRIK ELVANG
Publication year - 2011
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2010.02700.x
Subject(s) - staphylococcus aureus , osteomyelitis , microbiology and biotechnology , medicine , staphylococcal infections , biology , bacteria , immunology , genetics
Johansen LK, Frees D, Aalbæk B, Koch J, Iburg T, Nielsen OL, Leifsson PS, Jensen HE. A porcine model of acute, haematogenous, localized osteomyelitis due to Staphylococcus aureus : a pathomorphological study, APMIS 2010; 119: 111–8. A porcine model of acute, haematogenous, localized osteomyelitis was established. Serial dilutions of Staphylococcus aureus [5–50–500–5000–50 000 CFU/kg body weight (BW) suspended in saline or saline alone] were inoculated into the right brachial artery of pigs (BW 15 kg) separated into six groups of two animals. During the infection, blood was collected for cultivation, and after the animals were killed from day 5 to 15, they were necropsied and tissues were sampled for histopathology. Animals receiving ≤500 CFU/kg BW were free of lesions. Pigs inoculated with 5000 and 50 000 CFU/kg BW only developed microabscesses in bones of the infected legs. In the centre of microabscesses, S. aureus was regularly demonstrated together with necrotic neutrophils. Often, bone lesions resulted in trabecular osteonecrosis. The present localized model of acute haematogenous osteomyelitis revealed a pattern of development and presence of lesions similar to the situation in children. Therefore, this model should be reliably applied in studies of this disease with respect to e.g. pathophysiology and pathomorphology. Moreover, because of the regional containment of the infection to a defined number of bones, the model should be applicable also for screening of new therapy strategies.