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Exacerbation of Leishmania (Viannia) shawi infection in BALB/c mice after immunization with soluble antigen from amastigote forms
Author(s) -
PASSERO LUIZ FELIPE DOMINGUES,
DA COSTA BORDON MARIA LUIZA ANTUNES,
DE CARVALHO ANA KELY,
MARTINS LUCIANO MOURA,
CORBETT CARLOS EDUARDO PEREIRA,
LAURENTI MÁRCIA DALASTRA
Publication year - 2010
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2010.02679.x
Subject(s) - immune system , amastigote , immunology , antigen , biology , leishmania , immunization , lymph node , parasite hosting , world wide web , computer science
Passero LFD, da Costa Bordon MLA, de Carvalho AK, Martins LM, Corbett CEP, Laurenti MD. Exacerbation of Leishmania (Viannia) shawi infection in BALB/c mice after immunization with soluble antigen from amastigote forms. APMIS 2010; 118: 973–81. The present study aimed to evaluate the effects of immunization with soluble amastigote (AmaAg) and promastigote (ProAg) antigens from Leishmania (Viannia) shawi on the course of infection in BALB/c mice. After immunization with AmaAg, the challenged group showed greater lesion size and parasite load in the skin and lymph nodes, associated with diminished interleukin (IL)‐2, IL‐4, IL‐10, interferon (IFN)‐γ and nitrate levels in the supernatant of lymph node cell cultures, together with increases in transforming growth factor (TGF)‐β concentrations and humoral immune response. In contrast, immunization with ProAg led to smaller lesion size with reduced numbers of viable parasites in the skin. Protection was associated with increases in IL‐12, IFN‐γ, TGF‐β and nitrates and decreases in IL‐4 and IL‐10 levels. Concerning humoral immune response, a significant reduction in anti‐leishmania immunoglobulin G was verified in the ProAg‐challenged group. Analysis of these results suggests that AmaAg induced a suppressive cellular immune response in mice, favouring the spread of infection, whereas ProAg induced partial protection associated with increased cellular immune response.