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Tyrosine kinase mutations in gastrointestinal stromal tumors in a nation‐wide study in Iceland
Author(s) -
TRYGGVASON GEIR,
HILMARSDOTTIR BYLGJA,
GUNNARSSON GUðMUNDUR H.,
JÓNSSON JÓN JÓHANNES,
JÓNASSON JÓN G.,
MAGNÚSSON MAGNÚS K.
Publication year - 2010
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2010.02643.x
Subject(s) - pdgfra , gist , exon , mutation , cancer research , biology , population , proto oncogene proteins c kit , microbiology and biotechnology , gastrointestinal tract , tyrosine kinase , stromal tumor , stromal cell , gene , genetics , medicine , signal transduction , biochemistry , environmental health , stem cell , haematopoiesis , stem cell factor
Tryggvason G, Hilmarsdottir B, Gunnarsson GH, Jónsson JJ, Jónasson JG, Magnússon MK. Tyrosine kinase mutations in gastrointestinal stromal tumors in a nation‐wide study in Iceland. APMIS 2010; 118: 648–56. Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the gastrointestinal tract. It is characterized by activating mutations in the tyrosine kinase genes c‐kit or PDGFRA. This study examined the mutation rate and type in a population‐based material. All gastrointestinal mesenchymal tumors over the years 1990–2004 were evaluated and GIST tumors identified using immunohistochemistry (c‐kit) and conventional pathologic parameters. Paraffin sections from all tumors were subjected to mutation analysis on exons 9, 11, 13 and 17 of the c‐kit gene and exons 12 and 18 of the PDGFRA gene. To screen for mutations, we used a highly sensitive conformation‐sensitive gel electrophoresis (CSGE) and to define the mutated alleles, we employed direct automated DNA sequencing. All c‐kit‐positive gastrointestinal mesenchymal tumors were entered into the study. Fifty‐six tumors from 55 patients were analyzed. Mutations were found in 52 tumors representing a 92.9% mutational rate. Most of the mutations were found in c‐kit exon 11 (76.8%), followed by c‐kit exon 9 (10.7%). PDGFRA mutations were only found in three tumors. No correlation of mutation type with biologic behavior was found. This population‐based study, using a sensitive CSGE method, identifies mutations in the great majority of patients with GIST.