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The prevalence of ESBL‐producing E. coli and Klebsiella strains in the Copenhagen area of Denmark
Author(s) -
KJERULF ANNE,
HANSEN DENNIS S.,
SANDVANG DORTHE,
HANSEN FRANK,
FRIMODTMØLLER NIELS
Publication year - 2008
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2008.00777.x
Subject(s) - klebsiella , cefoxitin , microbiology and biotechnology , cefpodoxime , cefotetan , biology , gentamicin , escherichia coli , cephalosporin , klebsiella pneumoniae , antibiotic resistance , imipenem , antibiotics , gene , bacteria , genetics , staphylococcus aureus
The main purpose of the study was to investigate the frequency of ESBL‐producing E. coli and Klebsiella strains in the Greater Copenhagen area.Four collections of strains were investigated: A) 380 consecutive E. coli and Klebsiella isolates primarily from urine, B) 200 gentamicin‐resistant E. coli and Klebsiella isolates primarily from urine, C) 210consecutive E. coli isolates from blood cultures, and D) 68cefuroxime‐resistant E. coli and Klebsiella isolates primarilyfrom urine. Only one strain per patient was included. Strains with a zonediameter for cefpodoxime ≤23 mm were tested by a phenotypic confirmatorytest for ESBL production and all screening test‐positive strains were examinedwith PCR and nucleotide sequencing in order to detect the following ESBL genes: ctx‐m , shv , tem and oxa . Strains resistant tocefoxitin were further examined with cefotetan±boronic acid in orderto detect AmpC. An ESBL gene was detected in 3/3 confirmatorytest‐positive isolates from collection A, in 14/17 from collection B,and in 41/48 from collection D. The distribution of isolates with theESBL and/or AmpC enzymes was as follows: CTX‐M (n=41), SHV(n=14), AmpC (n=9), CTX‐M and AmpC (n=2), SHVand AmpC (n=1). In conclusion, the frequency of ESBL‐producing E.coli and Klebsiella isolates was low in the Copenhagen area ofDenmark (0.8 %). The most common ESBL genes found in our study were ctx‐m and shv genes.