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Elevated matrilysin levels in bronchoalveolar lavage fluid do not distinguish idiopathic pulmonary fibrosis from other interstitial lung diseases
Author(s) -
VUORINEN KIRSI,
MYLLÄRNIEMI MARJUKKA,
LAMMI LAURI,
PIIRILÄ PÄIVI,
RYTILÄ PAULA,
SALMENKIVI KAISA,
KINNULA VUOKKO L.
Publication year - 2007
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2007.apm_697.x
Subject(s) - bronchoalveolar lavage , idiopathic pulmonary fibrosis , medicine , matrilysin , pathology , sarcoidosis , lung , interstitial lung disease , idiopathic interstitial pneumonia , usual interstitial pneumonia , pulmonary fibrosis , parenchyma , fibrosis , immunohistochemistry
Microarray studies have shown that matrilysin or matrix metalloproteinase (MMP)‐7 is highly upregulated in the lungs of patients with idiopathic pulmonary fibrosis (IPF), but MMP‐7 protein expression has not been systematically compared between IPF and other interstitial lung diseases. MMP‐7 levels in bronchoalveolar lavage fluid (BALF) were compared to corresponding samples from nonspecific interstitial pneumonia (NSIP), sarcoidosis, and healthy controls. MMP‐7 levels in the BALF were determined by ELISA and localization of MMP‐7 in the lung tissue by immunohistochemistry. MMP‐7 was similarly elevated in the BALF of all these disorders compared to healthy controls (p=0.007). Even control subjects with prolonged cough displayed a tendency towards elevated MMP‐7 expression. There was a negative correlation between BALF MMP‐7 levels and forced expiratory vital capacity (r=−0.348, p=0.02, n=42). In IPF lung, MMP‐7 immunoreactivity appeared predominantly in the fibrotic parenchyma and arterial wall. In sarcoidosis and NSIP, prominent MMP‐7 immunoreactivity was found in areas of inflammation. These results demonstrate that elevated BALF MMP‐7 is not restricted to IPF alone but is also observed in other interstitial lung diseases and cannot be used as a differential diagnostic marker for IPF.