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In vitro functional test of two subclasses of an anti‐RhD antibody produced by transient expression in COS cells
Author(s) -
NIELSEN LEIF KOFOED,
NORDERHAUG LARS,
SANDLIE INGER,
DZIEGIEL MORTEN HANEFELD
Publication year - 2006
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2006.apm_381.x
Subject(s) - antibody , recombinant dna , antigen , immunology , fetus , hemolytic disease of the newborn (abo) , virology , biology , in vitro , medicine , pregnancy , gene , genetics
For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti‐RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti‐RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti‐RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti‐RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.