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Molecular serotyping of Salmonella : identification of the phase 1 H antigen based on partial sequencing of the fliC gene
Author(s) -
SONNEHANSEN JACOB,
JENABIAN SALOOMEH M.
Publication year - 2005
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2005.apm_113505.x
Subject(s) - serotype , flagellin , antigen , salmonella , h antigen , biology , serology , typing , gene , strain (injury) , microbiology and biotechnology , antibody , genetics , bacteria , anatomy
The purpose of this study was to develop a simple and non‐labour‐intensive molecular method to identify the phase 1 H antigens of Salmonella. The variable region of the flagellin gene, fliC , from 96 Salmonella strains representing 51 different phase 1 H antigens was sequenced in one direction. Unique sequences were found for 45 of the 51 different antigens. We were not able to separate either H:z 42 from H:d; H:g, q from H:g, m, q; H:l, w from H:Rl, z 40 or H:l, (v),z 13 from H:l,z, 13 . Several phase 2 H antigens were found to be encoded by fliC . Polymorphism, at the subspecies level, was observed in fliC of H:b, H:d, H:z 10 , H:z and especially H:k. By this method we were also able to confirm that one monophasic strain possesses a new antigen, H:z 91 . This study shows that sequence‐based typing of the phase 1 H antigen of Salmonella is a good alternative to serotyping when strains are non‐typable by serological methods.