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Application of molecular genetic methods in diagnostics and epidemiology of food‐borne bacterial pathogens
Author(s) -
LUKINMAA SUSANNA,
NAKARI ULLAMAIJA,
EKLUND MARJUT,
SIITONEN ANJA
Publication year - 2004
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1600-0463.2004.apm11211-1213.x
Subject(s) - biology , campylobacter , salmonella , salmonella enterica , pulsed field gel electrophoresis , molecular epidemiology , listeria monocytogenes , yersinia enterocolitica , yersinia , molecular diagnostics , microbiology and biotechnology , computational biology , genetics , bacteria , gene , genotype
Salmonella enterica , Campylobacter and Yersinia species, Shiga toxin‐producing Escherichia coli (STEC), Listeria monocytogenes and Clostridium perfringens are the bacterial pathogens constituting the greatest burden of food‐borne disease in Finland. Several molecular genetic methods have been applied to diagnose, discriminate and survey these bacteria. PCR, PCR‐RFLP and PFGE are the most widely and successfully used. However, these methods are unable to replace conventional and internationally standardised phenotyping. Electronic database libraries of the different genomic profiles will enable continuous surveillance of infections and detection of possible infection clusters at an early stage. Furthermore, whole‐genome sequence data have opened up new insights into epidemiological surveillance. Laboratory‐based surveillance performed in a timely manner and exploiting adequate methods, and co‐operation at local, national and international levels are among the key elements in preventing food‐borne diseases. This paper reviews different applications of molecular genetic methods for investigating enteric bacterial pathogens and gives examples of the methods successfully used in diagnostics and epidemiological studies in Finland.