Open AccessRelation of crystallin and ischemia/Reperfusion injury model of the rat retina
Author(s) -
YANG YUNSIK,
JANG JEONGUN,
BAHK SONGCHUL,
KIM JAEDUCK,
CHUNG HUNTAEG
Publication year - 2007
Publication title -
acta ophthalmologica scandinavica
Language(s) - English
Resource type - Journals
eISSN - 1600-0420
pISSN - 1395-3907
DOI - 10.1111/j.1600-0420.2007.01062_3218.x
Subject(s) - medicine , hemin , oxidative stress , reperfusion injury , ischemia , retina , blot , extracellular , kinase , crystallin , microbiology and biotechnology , pathology , biochemistry , endocrinology , chemistry , heme , biology , enzyme , neuroscience , gene
Abstract Purpose: Ischemic damage of organs has hypoxic stress and reperfusion of the organs induces oxidative stress. Crystallin family is known as a protector of various kind of stress. We wanted to investigated crystallin expression and what kind of crystallin is associated to the ischemia/reperfusion injury (I/R injury) of retina. Methods: Twenty four Sprague‐Dawley (SD) rats were used in this study. I/R injury was made by clamping optic nerve with central retina artery for 30min and then set free. Groups were divided into normal control, 24h and 72h after I/R injury, 24h and 72h after I/R injury with pretreatment of Sn(IV) protoporphyrin IX dichloride(SnPP) and 24h, 48h and 72h after I/R injury with pretreatment of hemin. All of the vitreous bodies were obtained and two‐dimensional electrophoresis (2‐DE) was performed. Results: Twenty‐three spots were identified by MALDI‐TOF/MS. Anti alpha A‐, alpha B‐, beta‐, phospho (ser19)‐alpha B‐, phospho (ser45)‐alpha B‐, phospho (ser59)‐alpha B‐crystallin, extracellular signal‐regulated kinases (ERK1/2), phospho‐ERK1/2 and heme oxigenase‐1 (HO‐1) antibody were used for Western blotting. Beta‐crystallins were increased 1.7 fold and 1.3 fold in the groups of I/R injury and I/R injury with SnPP pretreatment, respectively. Interestingly, I/R injured vitreous body showed the increased ERK1/2. However, I/R injured vitreous body pretreated with hemin for 24 h showed highly phosphorylated ERK1/2. Conclusions: This result might indicate that crystallin family is related to I/R injury. Phosphorylation of ERK1/2 was related with the hemin pretreatment.