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Quantification and characterization of GABA‐ergic amacrine cells in the retina of GAD67‐GFP knock‐in mice
Author(s) -
May Christian Albrecht,
Nakamura Kouichi,
Fujiyama Fumino,
Yanagawa Yuchio
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1600-0420.2007.01054.x
Subject(s) - retina , glutamate decarboxylase , parvalbumin , ganglion cell layer , calbindin , inner nuclear layer , choline acetyltransferase , green fluorescent protein , calretinin , tyrosine hydroxylase , gaba transporter , microbiology and biotechnology , amacrine cell , biology , glutamate receptor , chemistry , neuroscience , immunohistochemistry , neurotransmitter , cholinergic , central nervous system , biochemistry , immunology , receptor , enzyme , dopamine , gene
. Purpose: Although the presence of γ‐aminobutyrate acid (GABA) in amacrine cells and its co‐localization with other neuronal substances is well known, there exists only little information about their quantitative distribution in the mouse eye. The aim of the present study was to characterize GABA‐ergic amacrine cells in the retina of the recently introduced glutamate decarboxylase 67‐green fluorescent protein (GAD67‐GFP) knock‐in mouse. Methods: Whole mounts of the retina were prepared and the GFP‐positive neurons quantified. Immunofluorescence staining was performed with antibodies against GABA, calbindin (CB), calretinin (CR), parvalbumin (PV), choline acetyl transferase (ChAT), tyrosine hydroxylase (TH), vesicular glutamate transporter (VGluT) 1, VGluT2 and VGluT3. Results: Displaced GABA‐ergic amacrine cells in the ganglion cell layer (GCL) showed a density of 1006 ± 170 cells/mm 2 . In the inner nuclear layer (INL), the density of amacrine cells was 8821 ± 448 cells/mm 2 in the central region and 6825 ± 408 cells/mm 2 in the peripheral region. GFP‐positive amacrine cells co‐localized with GABA (99%), CR (INL 18%, GCL 71.3%), CB (INL 6.3%), bNOS (INL 1%, GCL 4%), and ChAT (INL 17%, GCL 92.6%). No co‐localization was seen with antibodies against PV, TH, and VGluT 1‐3. Conclusions: This study presents the first quantitative data concerning the co‐localization of GABA‐ergic neurons in the mouse retina with various neuronal markers.