Open AccessUVB‐induced formation of (6–4) photoproducts in the rat corneal epithelium
Author(s) -
Estil S.,
Olsen W. M.,
Huitfeldt H. S.,
Haaskjold E.
Publication year - 1997
Publication title -
acta ophthalmologica scandinavica
Language(s) - English
Resource type - Journals
eISSN - 1600-0420
pISSN - 1395-3907
DOI - 10.1111/j.1600-0420.1997.tb00104.x
Subject(s) - corneal epithelium , epithelium , medicine , in vivo , erythema , ultraviolet a , dna , microbiology and biotechnology , pathology , dermatology , chemistry , biology , biochemistry , genetics
The induction of DNA photoproducts in rat corneal epithelium was studied after in vivo exposure to different doses of ultraviolet B light at 297 nm. Affinity‐purified antibodies with a major specificity against UV‐induced (6–4) photoproducts were used. The results indicate a dose dependent formation of (6–4) photoproducts. Even a minimal erythema dose (25 mJ/cm 2 ) produced (6–4) photoproducts, demonstrating that DNA damage occurs in corneal tissue following exposure to biologically relevant doses of UVB light.