Open AccessAssessment of paraoxonase 1, xanthine oxidase and glutathione peroxidase activities, nitric oxide and thiol levels in women with polycystic ovary syndrome
Author(s) -
BASKOL GULDEN,
AYGEN ERCAN,
ERDEM FERAY,
CANIKLIOĞLU AYŞEN,
NARIN FIGEN,
ŞAHIN YILMAZ,
KAYA TUGBA
Publication year - 2012
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1111/j.1600-0412.2011.01337.x
Subject(s) - polycystic ovary , pon1 , medicine , endocrinology , oxidative stress , paraoxonase , glutathione peroxidase , xanthine oxidase , antioxidant , thiol , lipid peroxidation , chemistry , superoxide dismutase , biochemistry , enzyme , insulin resistance , insulin , genotype , gene
Objective. To investigate whether there is any relation between oxidative stress and the antioxidant system in the development of polycystic ovary syndrome (PCOS) by measuring serum nitric oxide (NO) levels and xanthine oxidase (XO) activity (a generator of reactive oxygen species) and antioxidant status by measuring serum thiol levels and glutathione peroxidase (GSH Px ) and paraoxonase 1 (PON1) activities. Design. Prospective case–control study. Setting. University hospital in Turkey. Sample. Thirty women with polycystic ovary syndrome and 20 age‐ and sex‐matched healthy control subjects were included. Methods. Serum XO, PON1 and GSH Px activity and NO and thiol levels were determined by spectrophotometric methods. Main outcome measures. Activity of serum XO, PON1 and GSH, as well as NO and thiol levels. Results. Serum XO activities were higher in women with PCOS than in the control women ( p <0.001). The PON1 activity was lower in women with PCOS than in the control women ( p <0.001). No significant difference was found between NO and thiol levels and GSH Px activities of women with PCOS and the control women ( p >0.05). Serum PON1 activities were negatively correlated with serum XO activities and NO levels. Conclusion. Increased oxidant XO activity and decreased lipid antioxidant PON1 activity, along with the observed negative correlation between these parameters, suggests that women with PCOS are under oxidative stress and that there is XO‐mediated lipid peroxidation, which may be related to increased atherosclerosis seen in later life in such women.