Open AccessLack of microsatellite instability in squamous cell vulvar carcinoma
Author(s) -
BUJKO MATEUSZ,
KOWALEWSKA MAGDALENA,
ZUB RENATA,
RADZISZEWSKI JAKUB,
BIDZINSKI MARIUSZ,
SIEDLECKI JANUSZ A.
Publication year - 2012
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1111/j.1600-0412.2011.01330.x
Subject(s) - microsatellite instability , microsatellite , polymerase chain reaction , pathology , vulvar carcinoma , carcinoma , medicine , epidermoid carcinoma , carcinogenesis , biology , cancer research , cancer , gene , genetics , allele
Mutator phenotypes with microsatellite instability (MSI) are observed in a subset of solid tumors. The objective of our study was to investigate the occurrence of MSI in vulvar squamous cell carcinoma (VSCC) and a possible relation between MSI and the presence of human papilloma virus (HPV). DNA samples from 44 tissue specimens of the primary VSCC as well as from six metastatic lymph node samples were analysed and compared with matched reference DNA from blood samples. The MSI status was examined by polymerase chain reaction (PCR) using the Bethesda panel of five microsatellite markers. PCR products were analysed by fluorescent capillary electrophoresis. No microsatellite instability was detected in tumor samples or in metastatic lymph nodes from any of the VSCC patients examined. Microsatellite instability seems not to play a major role in the carcinogenesis of VSCC and is probably not associated with the HPV‐related genetic background of this neoplasm.