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Altered membrane fluidity and lipid raft composition in presenilin‐deficient cells
Author(s) -
Grimm M. O. W.,
Tschäpe J.A.,
Grimm H. S.,
Zinser E. G.,
Hartmann T.
Publication year - 2006
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.2006.00682.x
Subject(s) - lipid raft , ganglioside , membrane fluidity , cholesterol , presenilin , membrane , microbiology and biotechnology , chemistry , biochemistry , lipid metabolism , membrane lipids , biology , alzheimer's disease , medicine , disease
The pathology of Alzheimer's disease is closely connected with lipid metabolism. Processing of amyloid precursor protein (APP) is sensitive to membrane alterations in levels of cholesterol and gangliosides. As cholesterol and gangliosides are major components of rafts and BACE I and γ ‐secretase are supposed to be localized to rafts there might be a yet unknown biological function underlying this connection. Increasing evidence shows a close connection between cholesterol homeostasis and APP processing and A β production respectively. We measured membrane fluidity by anisotropy determination, isolated detergent resistant membrane (DRM) fractions from membrane preparations and determined cholesterol content of these fractions by a coupled enzymatic assay. We found membrane fluidity to be changed in mouse embryonic fibroblasts (MEF) PS1/2 ‐/‐ along with altered cholesterol content in DRM fraction of these cells. In addition, total ganglioside levels were enhanced in absence of presenilin (PS).