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Effects of mannitol and glycerol on cerebral energy metabolism in gerbils
Author(s) -
Tsuda Y.,
Kitadai M.,
Hatanaka Y.,
Izumi Y.
Publication year - 1998
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1998.tb07375.x
Subject(s) - phosphocreatine , mannitol , ischemia , glycerol , chemistry , intracellular ph , saline , pi , pentobarbital , metabolism , endocrinology , medicine , energy metabolism , biochemistry , intracellular
Objectives – To evaluate the effects of infusion with hyperosmolar solutions, mannitol and glycerol on the recovery of cerebral energy metabolism during ischemia and reperfusion in the gerbil brain. Materials and methods – Sequential changes in cerebral energy metabolism following 90‐min ischemia and up to 8 h after reperfusion were measured in 15 gerbils using 31 P nuclear magnetic resonance (NMR) spectroscopy after 60‐min infusion of 10% glycerol (0.5 g/kg; n =5), or 20% mannitol (1.0 g/kg; n =5), and compared with those gerbils receiving with saline ( n =5). Gerbils were anesthetized by intraperitoneal injection of pentobarbital. Forebrain ischemia was induced by clipping of bilateral common carotid arteries for 90 min and reperfused. NMR spectroscopy was measured by a 6.34‐Tesla JEOL spectrometer, before administration, 2, 4, 6, and 8 h after 90‐min ischemia and reperfusion. Areas of inorganic phosphate (Pi), phosphocreatine (PCr), and β‐ATP peaks were measured to calculate parameters of cerebral energy metabolism, i.e., PCr/Pi and β‐ATP/Pi ratios. Intracellular pH (pHi) was calculated from chemical shifts of Pi relative to PCr. Results – PHi was higher in the mannitol group than in the glycerol and saline groups ( P <0.05) 2 h after reperfusion. PCr/Pi ratio was higher 2, 4, and 8 h after reperfusion ( P <0.01, P <0.05, P <0.01) in the mannitol group; and 6 h after reperfusion ( P <0.05) in the glycerol group; than in the saline group. β‐ATP/Pi ratio was higher 2 and 8 h after reperfusion ( P <0.05) in the glycerol group; and 2 h after reperfusion ( P <0.01) in the mannitol group, than in the saline group. Conclusions – The mannitol group had improved pHi higher than the glycerol group 2 h after reperfusion ( P <0.05), while the glycerol group had improved β‐ATP/Pi ratio higher than the mannitol group 6 h after reperfusion ( P <0.05). Both mannitol and glycerol groups had improved parameters of cerebral energy metabolism during ischemia and up to 8 h after reperfusion in the gerbil brain.