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Genetic susceptibility to multiple sclerosis in Sardinians: an immunological study
Author(s) -
Sotgiu S.,
Serra C.,
Marrosu M. G.,
Dolei A.,
Pugliatti M.,
Murgia B.,
Aiello I.,
Rosati G.
Publication year - 1998
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1998.tb01740.x
Subject(s) - peripheral blood mononuclear cell , multiple sclerosis , immunology , human leukocyte antigen , immune system , allele , in vitro , biology , genetic predisposition , tumor necrosis factor alpha , medicine , gene , genetics , antigen
Objectives ‐ We studied the in vitro production of variably MS‐related cytokines from Sardinian MS and healthy donors bearing the two “Sardinian” MS‐associated HLA‐DR alleles: DR3 and DR4 , with the purpose to evidentiate possible differences in their immune response. Materials and methods ‐ ELISA were used for detection of cellular products by mitogen‐activated peripheral blood mononuclear cells. Results ‐ PHA‐activated HLA‐DR4+/DR3‐ mononuclear cells produce significantly higher amounts of TNF‐α compared with the DR3 +/DR4‐ , In addition, homozygous HLA‐DR3+ mononuclear cells from MS patients produce significantly lower amounts of IL‐10 than those from homozygous HLA‐DR3+ healthy donors. Conclusion ‐ The abnormal production of detrimental or regulatory cytokines may account for the genetic susceptibility to MS in different HLA‐subgroups of Sardinian MS patients.