Premium
Suppressive effects of TNF‐α on myelin formation in vitro
Author(s) -
Tsukamoto T.,
Ishikawa M.,
Yamamoto T.
Publication year - 1995
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1995.tb05847.x
Subject(s) - remyelination , myelin , in vitro , tumor necrosis factor alpha , chemistry , immunology , biology , microbiology and biotechnology , endocrinology , biochemistry , central nervous system
Using organotypic cultures of ICR mouse cerebellum, we studied the effects of TNF‐α on myelin formation, and its demyelinating effects. Mature, myelinated cultures were resistant to TNF‐α, even at the highest concentration examined, 1 × 10 4 U/ml. However, when cultures were grown in medium containing TNF‐α from the time of explantation, myelin formation was significantly affected at a concentration of 1 × 10 2 U/ml. The myelin sheaths formed were very thin, partial, and few in number. When TNF‐α was removed from the medium within 6 days, myelin formation was recovered. Although our in vitro system tests factors that influence myelin formation but not remyelination, these results suggest that TNF‐α may be involved in the remyelination block following acute myelin breakdown.