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Specificity of intrathecal IgG synthesis for HTLV‐1 core and envelope proteins in HAM/TSP
Author(s) -
Kitze B.,
PuccioniSohler M.,
Schäffner J.,
Rieckmann P.,
Weber T.,
Felgenhauer K.,
Bodemer W.
Publication year - 1995
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1995.tb01690.x
Subject(s) - tropical spastic paraparesis , antigen , myelopathy , immunoassay , antibody , immunology , cerebrospinal fluid , intrathecal , immunoglobulin g , virology , medicine , virus , spinal cord , pathology , surgery , psychiatry
– In patients with human T‐cell lymphotropic virus type 1 (HTLV‐1) associated myelopathy/tropical spastic paraparesis (HAM/TSP), we investigated the significance of HTLV‐1 specific antibodies in the cerebrospinal fluid (CSF). Material and methods – The quantities of HTLV‐1 specific immunoglobulin G (IgG) in paired CSF and serum were evaluated by a sensitive enzyme immunoassay (EIA). The specificity of antiviral IgG was determined by radioimmunoprecipitation of HTLV‐1 antigens. Results – In 17 of 20 HAM/TSP patients, quantitative evaluation by EIA supplied evidence for antiviral IgG synthesis within the CNS. Radioimmunoprecipitation demonstrated IgG antibodies against HTLV‐1 envelope and core proteins in all HAM/TSP CSF and sera tested. Regarding the 3 sample pairs indeterminate in EIA for intrathecal synthesis, 2 showed stronger precipitation of HTLV‐1 antigens by CSF IgG than by equal amounts of serum IgG. Conclusion – Intrathecal antibody synthesis specific for both HTLV‐1 core and envelope antigens is common in HAM/TSP, thus providing conclusive evidence for an immune response to HTLV‐1 within the CNS.