Premium
Immunocytochemical detection of tumor necrosis factor‐α in infiltrating tumor cells in the cerebrospinal fluid from five patients with leptomeningeal carcinomatosis
Author(s) -
Nakamura S.,
Nagano I.,
Yoshioka M.,
Onodera J.,
Nakamura H.,
Shimazaki S.,
Tobita M.,
Itoyama Y.
Publication year - 1995
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1995.tb00420.x
Subject(s) - cerebrospinal fluid , pathology , tumor necrosis factor alpha , medicine , tumor cells , meningeal carcinomatosis , necrosis , cytoplasm , biology , cancer research , immunology , biochemistry
We studied the immunoreactivity for tumor necrosis factor‐α (TNF‐α) in cerebrospinal fluid (CSF) cell smears from five patients with leptomeningeal carcinomatosis (LC). Immunostained TNF‐α‐positive tumor cells had brownish deposits in the cytoplasm, but no deposits were observed in the nucleus. The intensity of the TNF‐α staining was greater in these tumor cells than in macrophages from patients with inflammatory diseases. Approximately 27–72% of the tumor cells were found to be TNF‐α immunoreactive in all five samples from the patient with LC. These results suggest that the large amount of TNF‐α produced by infiltrating tumor cells may play an important role in the development of the tumor.