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Search for an HTLV‐I‐like retrovirus in patients with MS by enzymatic DNA amplification
Author(s) -
Lisby G.
Publication year - 1993
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1993.tb05364.x
Subject(s) - retrovirus , polymerase chain reaction , peripheral blood mononuclear cell , virology , primer (cosmetics) , dna , virus , reverse transcriptase , homologous chromosome , microbiology and biotechnology , biology , enzyme , chemistry , genetics , gene , biochemistry , in vitro , organic chemistry
A retroviral etiology to MS has been sought for some time, so far reports of retroviral presence have not been confirmed by other groups. DNA was isolated from peripheral blood mononuclear cells from 67 patients with MS and brain capillaries from six patients with MS. Enzymatic amplification by the polymerase chain reaction was conducted with ten primersets homologous to highly conserved HTLV‐I/HTLV‐II genetic sequences at stringent and non‐stringent annealing conditions. No HTLV I/II related DNA fragments were seen judging from hybridization to an HTLV‐I probe, even at relaxed conditions. The present study does not support a role for a HTLV‐I‐like virus in MS.